BMRB: 10001

A high resolution structure of mastoparan-X strongly bound to lipid-bilayer membrane determined by solid-state NMR

Authors

Akutsu, H., Fujiwara, T., Todokoro, Y.

Assembly

mastoparan-X

Entity

1. mastoparan-X (polymer, Thiol state: not present), 14 monomers, 1555.970 Da Detail

INWKGIAAMA KKLX

Formula weight

1555.97 Da

Source organism

Vespa simillima xanthoptera

Exptl. method

solid-state NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 100.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	100.0 % (78 of 78)	100.0 % (63 of 63)	100.0 % (15 of 15)
Backbone	100.0 % (51 of 51)	100.0 % (38 of 38)	100.0 % (13 of 13)
Sidechain	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Aromatic	100.0 % (6 of 6)	100.0 % (5 of 5)	100.0 % (1 of 1)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. MP-X

INWKGIAAMA KKLX

Show sample condition

Sample

Temperature 253 (±0.1) K, pH 7.4 (±0.1), Details Mastoparan-X was bound with liposome (DPPC:DPPG=4:1).

#	Name	Isotope labeling	Type	Concentration
1	mastoparan-X	[U-95% 13C; U-95% 15N]		5 mg
2	liposome			50 mg

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Protein Blocks Logo

Calculated from 10 models in PDB: 2CZP, Strand ID: A Detail

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Release date

2015-09-02

Citation 1

Structure of tightly membrane-bound mastoparan-X, a G-protein-activating peptide, determined by solid-state NMR
Todokoro, Y., Yumen, I., Fukushima, K., Kang, S., Park, J., Kohno, T., Wakamatsu, K., Akutsu, H., Fujiwara, T.
Biophys. J. (2006), 91, 1368-1379, PubMed 16714348 , DOI 10.1529/biophysj.106.082735 , Abstract

Show more 1 citaion

Citation 2

Chemical shift referencing in MAS solid state NMR
Corey, M.R., Kurt, Z.W.
J. Magn. Reson. (2003), 162, 479-486, PubMed 12810033 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. mastoparan-X, : 1 : 2 entities Detail

Experiments performed 8 experiments Detail