BMRB: 10009

Solution structure of SH3 domain of mouse Kalirin-9a protein

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

SH3 domain of mouse Kalirin-9a protein

Entity

1. SH3 domain of mouse Kalirin-9a protein (polymer, Thiol state: all free), 74 monomers, 7633.405 Da Detail

GSSGSSGSTM TVIKDYYALK ENEICVSQGE VVQVLAVNQQ NMCLVYQPAS DHSPAAEGWV PGSILAPFSG PSSG

Formula weight

7633.405 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 92.9 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (731 of 787)	92.6 % (373 of 403)	94.8 % (290 of 306)	87.2 % (68 of 78)
Backbone	90.3 % (392 of 434)	88.1 % (133 of 151)	92.5 % (198 of 214)	88.4 % (61 of 69)
Sidechain	95.9 % (402 of 419)	95.2 % (240 of 252)	98.1 % (155 of 158)	77.8 % (7 of 9)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (24 of 24)	100.0 % (1 of 1)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. SH3 domain

GSSGSSGSTM TVIKDYYALK ENEICVSQGE VVQVLAVNQQ NMCLVYQPAS DHSPAAEGWV PGSILAPFSG PSSG

Show sample condition

Sample

Pressure 1 (±0.03) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFW, Strand ID: A Detail

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Release date

2006-11-05

Citation

Solution structure of SH3 domain of mouse Kalirin-9a protein
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
not known

Related entities 1. SH3 domain of mouse Kalirin-9a protein, : 1 : 134 entities Detail

Interaction partners 1. SH3 domain of mouse Kalirin-9a protein, : 17 interactors Detail

Experiments performed 2 experiments Detail