BMRB: 10013

Solution Structure Of The Ring Finger Domain Of The Human Kiaa1045 Protein

Authors

Miyamoto, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ring finger domain of KIAA1045 protein

Entity

1. RING Finger Domain (polymer, Thiol state: all other bound), 89 monomers, 9676.614 Da Detail

GSSGSSGPRE PVVNDEMCDV CEVWTAESLF PCRVCTRVFH DGCLRRMGYI QGDSAAEVTE MAHTETGWSC HYCDNINLLL TEESGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9807.433 Da

Max. entity weight

9676.614 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS40:ND1	2:ZN1:ZN
4	na	sing	1:CYS43:SG	2:ZN1:ZN
5	na	sing	1:CYS32:SG	2:ZN1:ZN
6	na	sing	1:CYS35:SG	2:ZN1:ZN
7	na	sing	1:CYS70:SG	2:ZN1:ZN
8	na	sing	1:CYS73:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 93.1 %, Completeness (bb): 89.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (874 of 939)	94.0 % (452 of 481)	93.7 % (344 of 367)	85.7 % (78 of 91)
Backbone	89.9 % (473 of 526)	90.2 % (165 of 183)	91.1 % (235 of 258)	85.9 % (73 of 85)
Sidechain	97.0 % (478 of 493)	96.3 % (287 of 298)	98.4 % (186 of 189)	83.3 % (5 of 6)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (34 of 34)	100.0 % (2 of 2)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. RING Finger Domain

GSSGSSGPRE PVVNDEMCDV CEVWTAESLF PCRVCTRVFH DGCLRRMGYI QGDSAAEVTE MAHTETGWSC HYCDNINLLL TEESGPSSG

Show sample condition

Sample

Pressure 1 (±0.03) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RING Finger Domain	[U-13C; U-15N]	protein	0.4 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	ZnCl2		salt	0.1 mM
7	D2O		solvent	10 %
8	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WIL, Strand ID: A Detail

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Release date

2006-12-05

Citation

Solution Structure of the RING Finger Domain of the Human KIAA1045 Protein
Miyamoto, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
not known

Related entities 1. RING Finger Domain, : 1 : 3 : 16 entities Detail

Interaction partners 1. RING Finger Domain, : 3 interactors Detail

Experiments performed 2 experiments Detail