BMRB: 10014

Assignments of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus

Authors

Okada, K., Takahashi, M., Sakamoto, T., Nakamura, K., Kanai, A., Kawai, G.

Assembly

helix6 in SRP RNA

Entity

1. helix6 in SRP RNA (polymer, Thiol state: not present), 12 monomers, 3969.372 Da Detail

GGCUGAAGGG CC

Formula weight

3969.372 Da

Source organism

Pyrococcus furiosus DSM 3638

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 60.2 %, Completeness (bb): 54.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	60.2 % (62 of 103)	60.2 % (62 of 103)
Suger, PO4	54.2 % (39 of 72)	54.2 % (39 of 72)
Nucleobase	74.2 % (23 of 31)	74.2 % (23 of 31)
Aromatic	92.0 % (23 of 25)	92.0 % (23 of 25)

1. pfHE6-12

GGCUGAAGGG CC

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Sample

Temperature 283 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	pfHE6-12			2.0 (±0.1) mM
2	sodium chloride			50 (±0.1) mM
3	phosphate buffer Na			20 (±0.1) mM

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Release date

2006-11-20

Citation

Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus
Okada, K., Takahashi, M., Sakamoto, T., Kawai, G., Nakamura, K., Kanai, A.
Nucleosides. Nucleotides. Nucleic Acids (2006), 25, 383-395, PubMed 16838833 , DOI 10.1080/15257770600683979 , Abstract

Related entities 1. helix6 in SRP RNA, : 1 entities Detail

Experiments performed 4 experiments Detail