BMRB: 10024

Chemical shift assignments for the Rhodobacter sphaeroides PufX membrane protein

Authors

Wang, Z., Suzuki, H., Kudo, M., Kobayashi, M., Nozawa, T.

Assembly

PufX

Entity

1. PufX (polymer, Thiol state: unknown), 77 monomers, 8639.864 Da Detail

ADKTIFNDHL NTNPKTNLRL WVAFQMMKGA GWAGGVFFGT LLLIGFFRVV GRMLPIQENQ APAPNITGAL EHHHHHH

Formula weight

8639.864 Da

Source organism

Cereibacter sphaeroides

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 58.4 %, Completeness (bb): 76.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	58.4 % (534 of 915)	65.8 % (310 of 471)	41.4 % (149 of 360)	89.3 % (75 of 84)
Backbone	76.4 % (347 of 454)	88.6 % (140 of 158)	62.8 % (140 of 223)	91.8 % (67 of 73)
Sidechain	47.4 % (251 of 530)	54.3 % (170 of 313)	35.4 % (73 of 206)	72.7 % (8 of 11)
Aromatic	8.9 % (10 of 112)	8.9 % (5 of 56)	9.3 % (5 of 54)	0.0 % (0 of 2)
Methyl	40.9 % (36 of 88)	61.4 % (27 of 44)	20.5 % (9 of 44)

1. PufX polypeptide

ADKTIFNDHL NTNPKTNLRL WVAFQMMKGA GWAGGVFFGT LLLIGFFRVV GRMLPIQENQ APAPNITGAL EHHHHHH

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	PufX polypeptide	[U-13C; U-15N]	protein	2 mM
2	Chloroform		solvent	50 %
3	Methanol		solvent	50 %

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Protein Blocks Logo

Calculated from 7 models in PDB: 2DW3, Strand ID: A Detail

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Release date

2007-06-12

Citation

Solution structure of the Rhodobacter sphaeroides PufX membrane protein: implications for the quinone exchange and protein-protein interactions
Wang, Z., Suzuki, H., Kobayashi, M., Nozawa, T.
Biochemistry (2007), 46, 3635-3642, PubMed 17335288 , DOI 10.1021/bi0618060 , Abstract

Related entities 1. PufX, : 1 : 13 entities Detail

Experiments performed 7 experiments Detail