BMRB: 10025

Solution structure of kinase associated domain 1 of mouse MAP/microtubule affinity-regulating kinase 3

Authors

Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

MAP/microtubule affinity-regulating kinase 3

Entity

1. MAP/microtubule affinity-regulating kinase 3 (polymer, Thiol state: all free), 102 monomers, 11516.11 Da Detail

GSSGSSGRFT WSMKTTSSMD PSDMMREIRK VLGANNCDYE QRERFLLFCV HGDGHAENLV QWEMEVCKLP RLSLNGVRFK RISGTSIAFK NIASKIANEL KL

Formula weight

11516.11 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 95.4 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1136 of 1191)	95.4 % (595 of 624)	95.8 % (438 of 457)	93.6 % (103 of 110)
Backbone	93.9 % (571 of 608)	93.8 % (197 of 210)	94.3 % (281 of 298)	93.0 % (93 of 100)
Sidechain	96.9 % (656 of 677)	96.1 % (398 of 414)	98.0 % (248 of 253)	100.0 % (10 of 10)
Aromatic	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (43 of 43)	100.0 % (2 of 2)
Methyl	95.7 % (90 of 94)	95.7 % (45 of 47)	95.7 % (45 of 47)

1. kinase associated domain 1(KA1)

GSSGSSGRFT WSMKTTSSMD PSDMMREIRK VLGANNCDYE QRERFLLFCV HGDGHAENLV QWEMEVCKLP RLSLNGVRFK RISGTSIAFK NIASKIANEL KL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	KA1 domain	[U-13C; U-15N]	protein	1.0 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UL7, Strand ID: A Detail

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Release date

2008-07-15

Citation

Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3
Tochio, N., Koshiba, S., Kobayashi, N., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Tomo, Y., Motoda, Y., Kobayashi, A., Tanaka, A., Hayashizaki, Y., Terada, T., Shirouzu, M., Kigawa, T., Yokoyama, S.
Protein Sci. (2006), 15, 2534-2543, PubMed 17075132 , DOI 10.1110/ps.062391106 , Abstract

Related entities 1. MAP/microtubule affinity-regulating kinase 3, : 1 : 4 : 48 entities Detail

Interaction partners 1. MAP/microtubule affinity-regulating kinase 3, : 1 interactors Detail

Experiments performed 2 experiments Detail