BMRB: 10026

Solution structure of kinase associated domain 1 of mouse MAP/microtubule affinity-regulating kinase 3

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

MAP/microtubule affinity-regulating kinase 3

Entity

1. MAP/microtubule affinity-regulating kinase 3 (polymer, Thiol state: all free), 126 monomers, 14252.95 Da Detail

MKDHLIHNVH KEEHAHAHNK DYDIPTTENL YFQGSSGSSG DMMREIRKVL GANNCDYEQR ERFLLFCVHG DGHAENLVQW EMEVCKLPRL SLNGVRFKRI SGTSIAFKNI ASKIANELKL SGPSSG

Formula weight

14252.95 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 98.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.0 % (1436 of 1465)	96.7 % (741 of 766)	99.5 % (560 of 563)	99.3 % (135 of 136)
Backbone	98.8 % (741 of 750)	97.3 % (252 of 259)	99.7 % (367 of 368)	99.2 % (122 of 123)
Sidechain	97.6 % (811 of 831)	96.4 % (489 of 507)	99.4 % (309 of 311)	100.0 % (13 of 13)
Aromatic	96.6 % (114 of 118)	96.6 % (57 of 59)	96.6 % (56 of 58)	100.0 % (1 of 1)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. Kinase associated domain 1 (KA1)

MKDHLIHNVH KEEHAHAHNK DYDIPTTENL YFQGSSGSSG DMMREIRKVL GANNCDYEQR ERFLLFCVHG DGHAENLVQW EMEVCKLPRL SLNGVRFKRI SGTSIAFKNI ASKIANELKL SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	KA1 domain	[U-13C; U-15N]	protein	2.0 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1V5S, Strand ID: A Detail

---

Release date

2008-06-23

Citation

Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3
Tochio, N., Koshiba, S., Kobayashi, N., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Tomo, Y., Motoda, Y., Kobayashi, A., Tanaka, A., Hayashizaki, Y., Terada, T., Shirouzu, M., Kigawa, T., Yokoyama, S.
Protein Sci. (2006), 15, 2534-2543, PubMed 17075132 , DOI 10.1110/ps.062391106 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. MAP/microtubule affinity-regulating kinase 3, : 1 : 10 entities Detail

Experiments performed 2 experiments Detail