BMRBj - BMREntryMaster

Found 1 Document (1.046 seconds)

BMRB: 10027

2E0G

chemical shift assignment of DnaA domain I-II

Authors

Abe, Y., Watanabe, N., Yoshida, Y., Katayama, T., Ueda, T.

Assembly

DnaA

Entity

1. DnaA (polymer, Thiol state: all free), 107 monomers, 11916.37 Da Detail

SLSLWQQCLA RLQDELPATE FSMWIRPLQA ELSDNTLALY APNRFVLDWV RDKYLNNING LLTSFCGADA PQLRFEVGTK PVTQTPQAAV TSNVAAPAQV AQTQPQR

Formula weight

11916.37 Da

Source organism

Escherichia coli K-12

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 92.9 %, Completeness (bb): 96.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (1159 of 1247)	92.1 % (595 of 646)	94.4 % (455 of 482)	91.6 % (109 of 119)
Backbone	96.5 % (604 of 626)	95.7 % (200 of 209)	96.9 % (308 of 318)	97.0 % (96 of 99)
Sidechain	90.1 % (653 of 725)	89.5 % (391 of 437)	92.9 % (249 of 268)	65.0 % (13 of 20)
Aromatic	90.2 % (83 of 92)	91.3 % (42 of 46)	88.4 % (38 of 43)	100.0 % (3 of 3)
Methyl	91.8 % (123 of 134)	94.0 % (63 of 67)	89.6 % (60 of 67)

1. N-terminal domain of DnaA

SLSLWQQCLA RLQDELPATE FSMWIRPLQA ELSDNTLALY APNRFVLDWV RDKYLNNING LLTSFCGADA PQLRFEVGTK PVTQTPQAAV TSNVAAPAQV AQTQPQR

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Referencing offset: 0.18 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2E0G, Strand ID: A Detail

Release date

2007-06-25

Citation

Assignment of 1H, 13C and 15N resonances of N-terminal domain of DnaA protein
Yoshito, A., Noriko, W., Yuichiro, Y., Fumi, E., Tsutomu, K., Tadashi, U.
Biomol. NMR Assign. (2007), 1, 57-59, PubMed 19636826 , DOI 10.1007/s12104-007-9015-2 , Abstract

Related entities 1. DnaA, : 1 : 5 : 2 : 67 entities Detail

Interaction partners 1. DnaA, : 14 interactors Detail

More details for 14 interactors identified by 8 citations

Experiments performed 15 experiments Detail

1 1H-15N HSQC

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

2 1H-13C HSQC

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

3 HNCA

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

4 HNCOCA

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

5 HNCACO

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

6 HNCO

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

7 CBCACONH

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

8 HNCACB

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

9 15N 3D TOCSY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

10 15N enhanced NOESY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

11 HCCH TOCSY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

12 HCCH COSY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

13 13C enhanced NOESY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

14 1H NOESY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

15 1H TOCSY

Instrument

Varian Inova 600 - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of DnaA	[U-13C; U-15N]	protein	0.5 (±0.2) mM
2	sodium phosphate		buffer	50 mM
3	ethylenediaminetetracetic acid		additive	1 mM
4	sodium chloride		salt	40 mM
5	Dithiothreitol		additive	2 mM
6	sucrose		additive	10 %

nullKeywords N-terminal domain of DnaA, DnaA, replication initiation, replication initiation factor