BMRB: 10028

Solution structure of the second WW domain from mouse salvador homolog 1 protein (mm45).

Authors

Ohnishi, S., Kigawa, T., Koshiba, S., Tomizawa, T., Sato, M., Tochio, N., Inoue, M., Harada, T., Watanabe, S., Yokoyama, S.

Assembly

the second WW domain of mouse salvador homolog 1 protein (mWW45)

Entity

1. the second WW domain of mouse salvador homolog 1 protein (mWW45) (polymer, Thiol state: all free), 49 monomers, 5294.549 × 2 Da Detail

GSSGSSGPLE REGLPPGWER VESSEFGTYY VDHTNKRAQY RHPSGPSSG

Total weight

10589.098 Da

Max. entity weight

5294.549 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.8 %, Completeness: 88.5 %, Completeness (bb): 84.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.5 % (469 of 530)	88.2 % (246 of 279)	90.2 % (184 of 204)	83.0 % (39 of 47)
Backbone	84.2 % (239 of 284)	83.2 % (84 of 101)	85.6 % (119 of 139)	81.8 % (36 of 44)
Sidechain	93.4 % (268 of 287)	91.0 % (162 of 178)	97.2 % (103 of 106)	100.0 % (3 of 3)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)

1. WW domain

GSSGSSGPLE REGLPPGWER VESSEFGTYY VDHTNKRAQY RHPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.03) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	WW domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		reducing agent	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DWV, Strand ID: A, B Detail

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Release date

2007-10-18

Citation

Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form
Ohnishi, S., Guntert, P., Koshiba, S., Tomizawa, T., Akasaka, R., Tochio, N., Sato, M., Inoue, M., Harada, T., Watanabe, S., Tanaka, A., Shirouzu, M., Kigawa, T., Yokoyama, S.
FEBS Lett. (2007), 581, 462-468, PubMed 17239860 , DOI 10.1016/j.febslet.2007.01.008 , Abstract

Related entities 1. the second WW domain of mouse salvador homolog 1 protein (mWW45), : 1 : 125 entities Detail

Experiments performed 2 experiments Detail