BMRB: 10029

Solution structure of the fifth PDZ domain of human membrane associated guanylate kinase inverted-2 (KIAA0705 protein)

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

membrane associated guanylate kinase inverted-2

Entity

1. membrane associated guanylate kinase inverted-2 (polymer, Thiol state: all free), 103 monomers, 11170.47 Da Detail

GSSGSSGQDF DYFTVDMEKG AKGFGFSIRG GREYKMDLYV LRLAEDGPAI RNGRMRVGDQ IIEINGESTR DMTHARAIEL IKSGGRRVRL LLKRGTGSGP SSG

Formula weight

11170.47 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 94.1 %, Completeness (bb): 92.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.1 % (1076 of 1144)	93.2 % (560 of 601)	95.4 % (418 of 438)	93.3 % (98 of 105)
Backbone	92.7 % (569 of 614)	91.9 % (204 of 222)	93.1 % (271 of 291)	93.1 % (94 of 101)
Sidechain	95.6 % (588 of 615)	93.9 % (356 of 379)	98.3 % (228 of 232)	100.0 % (4 of 4)
Aromatic	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. PDZ domain

GSSGSSGQDF DYFTVDMEKG AKGFGFSIRG GREYKMDLYV LRLAEDGPAI RNGRMRVGDQ IIEINGESTR DMTHARAIEL IKSGGRRVRL LLKRGTGSGP SSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.3 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 6 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFV, Strand ID: A Detail

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Release date

2008-08-11

Citation

Solution structure of the fifth PDZ domain of human membrane associated guanylate kinase inverted-2 (KIAA0705 protein)
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. membrane associated guanylate kinase inverted-2, : 1 : 6 : 389 entities Detail

Interaction partners 1. membrane associated guanylate kinase inverted-2, : 3 interactors Detail

Experiments performed 2 experiments Detail