BMRB: 10030

Solution structure of the forth CH domain from human plastin 3 T-isoform

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

T-plastin

Entity

1. T-plastin (polymer, Thiol state: all free), 124 monomers, 13239.96 Da Detail

GSSGSSGNDD IIVNWVNRTL SEAGKSTSIQ SFKDKTISSS LAVVDLIDAI QPGCINYDLV KSGNLTEDDK HNNAKYAVSM ARRIGARVYA LPEDLVEVKP KMVMTVFACL MGRGMKRVSG PSSG

Formula weight

13239.96 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 96.8 %, Completeness (bb): 96.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.8 % (1323 of 1367)	95.8 % (677 of 707)	98.1 % (520 of 530)	96.9 % (126 of 130)
Backbone	96.2 % (708 of 736)	94.5 % (241 of 255)	97.2 % (351 of 361)	96.7 % (116 of 120)
Sidechain	97.6 % (726 of 744)	96.5 % (436 of 452)	99.3 % (280 of 282)	100.0 % (10 of 10)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (29 of 29)	100.0 % (1 of 1)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. CH domain

GSSGSSGNDD IIVNWVNRTL SEAGKSTSIQ SFKDKTISSS LAVVDLIDAI QPGCINYDLV KSGNLTEDDK HNNAKYAVSM ARRIGARVYA LPEDLVEVKP KMVMTVFACL MGRGMKRVSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]		1.16 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.11 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJO, Strand ID: A Detail

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Release date

2008-08-11

Citation

Solution structure of the forth CH domain from human plastin 3 T-isoform
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. T-plastin, : 1 : 9 : 108 entities Detail

Interaction partners 1. T-plastin, : 6 interactors Detail

Experiments performed 2 experiments Detail