BMRB: 10032

Solution structure of the first mbt domain from human KIAA1798 protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

KIAA1798 protein

Entity

1. KIAA1798 protein (polymer, Thiol state: all free), 127 monomers, 14023.54 Da Detail

GSSGSSGPYN KNGFKVGMKL EGVDPEHQSV YCVLTVAEVC GYRIKLHFDG YSDCYDFWVN ADALDIHPVG WCEKTGHKLH PPKGYKEEEF NWQTYLKTCK AQAAPKSLFE NQNITVIPSG FSGPSSG

Formula weight

14023.54 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 93.3 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (1363 of 1461)	91.7 % (695 of 758)	95.8 % (547 of 571)	91.7 % (121 of 132)
Backbone	92.4 % (689 of 746)	89.6 % (233 of 260)	94.3 % (346 of 367)	92.4 % (110 of 119)
Sidechain	94.6 % (783 of 828)	92.8 % (462 of 498)	97.8 % (310 of 317)	84.6 % (11 of 13)
Aromatic	98.8 % (170 of 172)	97.7 % (84 of 86)	100.0 % (83 of 83)	100.0 % (3 of 3)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. mbt domain

GSSGSSGPYN KNGFKVGMKL EGVDPEHQSV YCVLTVAEVC GYRIKLHFDG YSDCYDFWVN ADALDIHPVG WCEKTGHKLH PPKGYKEEEF NWQTYLKTCK AQAAPKSLFE NQNITVIPSG FSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	mbt domain	[U-13C; U-15N]		1.03 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJS, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution NMR structure of the DNA-binding domain from Scml2 (sex comb on midleg-like 2)
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
J. Biol. Chem. (2014), 289, 15739-15749, PubMed 24727478 , DOI 10.1074/jbc.M113.524009 , Abstract

Related entities 1. KIAA1798 protein, : 1 : 5 : 89 entities Detail

Interaction partners 1. KIAA1798 protein, : 40 interactors Detail

Experiments performed 2 experiments Detail