BMRB: 10033

Soluiotn structure of J-domain of mouse DnaJ like protein

Authors

Kobayashi, N., Koshiba, S., Inoue, M., Tochio, N., Tomizawa, T., Kigawa, T., Yokoyama, S.

Assembly

1700030A21Rik protein

Entity

1. 1700030A21Rik protein (polymer, Thiol state: all free), 94 monomers, 10442.69 Da Detail

GSSGSSGMAL EQTLKKDWYS ILGADPSANM SDLKQKYQKL ILLYHPDKQS ADVPAGTMEE CMQKFIEIDQ AWKILGNEET KKKYDLQRSG PSSG

Formula weight

10442.69 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 96.3 %, Completeness (bb): 94.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1057 of 1098)	96.0 % (556 of 579)	97.1 % (406 of 418)	94.1 % (95 of 101)
Backbone	94.1 % (523 of 556)	92.2 % (177 of 192)	95.6 % (262 of 274)	93.3 % (84 of 90)
Sidechain	98.4 % (618 of 628)	97.9 % (379 of 387)	99.1 % (228 of 230)	100.0 % (11 of 11)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (33 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. J-domain

GSSGSSGMAL EQTLKKDWYS ILGADPSANM SDLKQKYQKL ILLYHPDKQS ADVPAGTMEE CMQKFIEIDQ AWKILGNEET KKKYDLQRSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	J-domain of DnaJ like protein	[U-13C; U-15N]		1.07 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJZ, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of J-domain of mouse DnaJ like protein
Kobayashi, N., Koshiba, S., Inoue, M., Tochio, N., Tomizawa, T., Kigawa, T., Yokoyama, S.

Related entities 1. 1700030A21Rik protein, : 1 : 2 : 68 entities Detail

Experiments performed 2 experiments Detail