Solution structure of Fibronectin type III domain derived from human KIAA0970 protein
GSSGSSGDEE TKAFEALLSN IVKPVASDIQ ARTVVLTWSP PSSLINGETD ESSVPELYGY EVLISSTGKD GKYKSVYVGE ETNITLNDLK PAMDYHAKVQ AEYNSIKGTP SEAEIFTTLS CEPDIPNPPR ISGPSSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 98.2 % (1472 of 1499) | 98.3 % (761 of 774) | 98.1 % (579 of 590) | 97.8 % (132 of 135) |
Backbone | 97.9 % (783 of 800) | 98.2 % (269 of 274) | 97.8 % (391 of 400) | 97.6 % (123 of 126) |
Sidechain | 98.7 % (814 of 825) | 98.4 % (492 of 500) | 99.1 % (313 of 316) | 100.0 % (9 of 9) |
Aromatic | 100.0 % (84 of 84) | 100.0 % (42 of 42) | 100.0 % (41 of 41) | 100.0 % (1 of 1) |
Methyl | 100.0 % (144 of 144) | 100.0 % (72 of 72) | 100.0 % (72 of 72) |
1. Fibronectin type3 domain
GSSGSSGDEE TKAFEALLSN IVKPVASDIQ ARTVVLTWSP PSSLINGETD ESSVPELYGY EVLISSTGKD GKYKSVYVGE ETNITLNDLK PAMDYHAKVQ AEYNSIKGTP SEAEIFTTLS CEPDIPNPPR ISGPSSGPressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | fibronectin type III domain | [U-13C; U-15N] | 1.11 mM | |
2 | Phosphate | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | 0.02 % | ||
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |