BMRB: 10035

Solution structure of Lectin C-type domain derived from a hypothetical protein from C. elegans

Authors

Kobayashi, N., Koshiba, S., Inoue, M., Tochio, N., Kigawa, T., Yokoyama, S.

Assembly

Hypothetical protein yk1067a12

Entity

1. Hypothetical protein yk1067a12 (polymer, Thiol state: all free), 150 monomers, 16404.66 Da Detail

GSSGSSGVKF LTVNDDILSM PQARNFCASA GGYLADDLGD DKNNFYSSIA ANTQFWIGLF KNSDGQFYWD RGQGINPDLL NQPITYWANG EPSNDPTRQC VYFDGRSGDK SKVWTTDTCA TPRPFICQKH RYDSDHKPNT IGDASGPSSG

Formula weight

16404.66 Da

Source organism

Caenorhabditis elegans

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 97.3 %, Completeness (bb): 96.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.3 % (1627 of 1672)	96.8 % (839 of 867)	98.0 % (629 of 642)	97.5 % (159 of 163)
Backbone	96.7 % (853 of 882)	95.4 % (293 of 307)	97.5 % (423 of 434)	97.2 % (137 of 141)
Sidechain	97.9 % (905 of 924)	97.5 % (546 of 560)	98.5 % (337 of 342)	100.0 % (22 of 22)
Aromatic	99.5 % (183 of 184)	100.0 % (92 of 92)	98.9 % (87 of 88)	100.0 % (4 of 4)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. Lectin C-type homologue domain

GSSGSSGVKF LTVNDDILSM PQARNFCASA GGYLADDLGD DKNNFYSSIA ANTQFWIGLF KNSDGQFYWD RGQGINPDLL NQPITYWANG EPSNDPTRQC VYFDGRSGDK SKVWTTDTCA TPRPFICQKH RYDSDHKPNT IGDASGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Lectin C-type domain	[U-13C; U-15N]		0.89 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WK1, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution structure of Lectin C-type domain derived from a hypothetical protein from C. elegans
Kobayashi, N., Koshiba, S., Inoue, M., Tochio, N., Kigawa, T., Yokoyama, S.

Related entities 1. Hypothetical protein yk1067a12, : 1 : 87 entities Detail

Experiments performed 2 experiments Detail