BMRB: 10036

Solution structure of mouse CGI-38 protein

Authors

Kobayashi, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Protein CGI-38

Entity

1. Protein CGI-38 (polymer, Thiol state: all free), 151 monomers, 15921.77 Da Detail

GSSGSSGMAA STDIAGLEES FRKFAIHGDP KASGQEMNGK NWAKLCKDCK VADGKAVTGT DVDIVFSKVK AKSARVINYE EFKKALEELA TKRFKGKSKE EAFDAICQLI AGKEPANIGV TKAKTGGAVD RLTDTSKYTG SHKERSGPSS G

Formula weight

15921.77 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.8 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.8 % (1630 of 1666)	97.0 % (842 of 868)	98.4 % (633 of 643)	100.0 % (155 of 155)
Backbone	98.9 % (890 of 900)	98.7 % (312 of 316)	98.6 % (430 of 436)	100.0 % (148 of 148)
Sidechain	97.1 % (874 of 900)	96.0 % (530 of 552)	98.8 % (337 of 341)	100.0 % (7 of 7)
Aromatic	91.7 % (88 of 96)	91.7 % (44 of 48)	91.5 % (43 of 47)	100.0 % (1 of 1)
Methyl	99.3 % (137 of 138)	98.6 % (68 of 69)	100.0 % (69 of 69)

1. Protein CGI-38

GSSGSSGMAA STDIAGLEES FRKFAIHGDP KASGQEMNGK NWAKLCKDCK VADGKAVTGT DVDIVFSKVK AKSARVINYE EFKKALEELA TKRFKGKSKE EAFDAICQLI AGKEPANIGV TKAKTGGAVD RLTDTSKYTG SHKERSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CGI-38	[U-13C; U-15N]		1.23 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WLM, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of mouse CGI-38 protein
Kobayashi, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Protein CGI-38, : 1 : 2 : 30 entities Detail

Interaction partners 1. Protein CGI-38, : 4 interactors Detail

Experiments performed 2 experiments Detail