BMRB: 10037

Solution structure of the third mbt domain from human KIAA1798 protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

KIAA1798 protein

Entity

1. KIAA1798 protein (polymer, Thiol state: all free), 107 monomers, 11571.78 Da Detail

GSSGSSGVKP PHGFQKKMKL EVVDKRNPMF IRVATVADTD DHRVKVHFDG WNNCYDYWID ADSPDIHPVG WCSKTGHPLQ PPLSPLELME ASEHGGCSTP GSGPSSG

Formula weight

11571.78 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 92.8 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.8 % (1101 of 1186)	91.4 % (565 of 618)	94.8 % (440 of 464)	92.3 % (96 of 104)
Backbone	91.5 % (567 of 620)	87.9 % (189 of 215)	93.9 % (290 of 309)	91.7 % (88 of 96)
Sidechain	94.6 % (625 of 661)	93.3 % (376 of 403)	96.4 % (241 of 250)	100.0 % (8 of 8)
Aromatic	96.2 % (102 of 106)	96.2 % (51 of 53)	96.0 % (48 of 50)	100.0 % (3 of 3)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. mbt domain

GSSGSSGVKP PHGFQKKMKL EVVDKRNPMF IRVATVADTD DHRVKVHFDG WNNCYDYWID ADSPDIHPVG WCSKTGHPLQ PPLSPLELME ASEHGGCSTP GSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	mbt domain	[U-13C; U-15N]		1.18 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJQ, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of the third mbt domain from human KIAA1798 protein
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. KIAA1798 protein, : 1 : 3 : 40 entities Detail

Interaction partners 1. KIAA1798 protein, : 41 interactors Detail

Experiments performed 2 experiments Detail