BMRB: 10038

Solution structure of zf-C2H2 domains from human Zinc finger protein 295

Authors

Tomizawa, T., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Zinc finger protein 295

Entity

1. zf-C2H2 domain (polymer, Thiol state: all free), 107 monomers, 11963.38 Da Detail

GSSGSSGASP VENKEVYQCR LCNAKLSSLL EQGSHERLCR NAAVCPYCSL RFFSPELKQE HESKCEYKKL TCLECMRTFK SSFSIWRHQV EVHNQNNMAP TSGPSSG

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

12159.606 Da

Max. entity weight

11963.38 Da

Entity Connection

na 12 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS19:SG	2:ZN1:ZN
2	na	sing	1:CYS22:SG	2:ZN1:ZN
3	na	sing	1:HIS35:NE2	2:ZN1:ZN
4	na	sing	1:CYS39:SG	2:ZN1:ZN
5	na	sing	1:CYS45:SG	2:ZN1:ZN
6	na	sing	1:CYS48:SG	2:ZN1:ZN
7	na	sing	1:HIS61:NE2	2:ZN1:ZN
8	na	sing	1:CYS65:SG	2:ZN1:ZN
9	na	sing	1:CYS72:SG	2:ZN1:ZN
10	na	sing	1:CYS75:SG	2:ZN1:ZN
11	na	sing	1:HIS88:NE2	2:ZN1:ZN
12	na	sing	1:HIS93:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 95.4 %, Completeness (bb): 94.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1166 of 1222)	94.9 % (610 of 643)	96.6 % (449 of 465)	93.9 % (107 of 114)
Backbone	94.1 % (595 of 632)	93.0 % (200 of 215)	95.2 % (300 of 315)	93.1 % (95 of 102)
Sidechain	96.7 % (668 of 691)	95.8 % (410 of 428)	98.0 % (246 of 251)	100.0 % (12 of 12)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (45 of 45)	100.0 % (1 of 1)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. zf-C2H2 domain

GSSGSSGASP VENKEVYQCR LCNAKLSSLL EQGSHERLCR NAAVCPYCSL RFFSPELKQE HESKCEYKKL TCLECMRTFK SSFSIWRHQV EVHNQNNMAP TSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]		1.03 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	ZnCl2			0.01 mM
6	NaN3			0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJP, Strand ID: A Detail

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Release date

2008-08-11

Citation

Solution structure of zf-C2H2 domains from human Zinc finger protein 295
Tomizawa, T., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 2 : 33 entities Detail

Interaction partners 1. zf-C2H2 domain, : 6 interactors Detail

Experiments performed 2 experiments Detail