BMRB: 10039

Solution Structure of the Pleckstrin Homology Domain of Mouse Ethanol Decreased 4 Protein

Authors

Li, H., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

FYVE, RhoGEF and PH domain containing 6; ethanol decreased 4

Entity

1. FYVE, RhoGEF and PH domain containing 6; ethanol decreased 4 (polymer, Thiol state: all free), 109 monomers, 12001.36 Da Detail

GSSGSSGSTM SGYLYRSKGS KKPWKHLWFV IKNKVLYTYA ASEDVAALES QPLLGFTVTL VKDENSESKV FQLLHKGMVF YVFKADDAHS TQRWIDAFQE GTVSGPSSG

Formula weight

12001.36 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 96.4 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1218 of 1263)	95.7 % (622 of 650)	97.4 % (485 of 498)	96.5 % (111 of 115)
Backbone	95.5 % (619 of 648)	93.8 % (211 of 225)	96.5 % (306 of 317)	96.2 % (102 of 106)
Sidechain	97.3 % (695 of 714)	96.7 % (411 of 425)	98.2 % (275 of 280)	100.0 % (9 of 9)
Aromatic	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (71 of 71)	100.0 % (3 of 3)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. Pleckstrin Homology domain

GSSGSSGSTM SGYLYRSKGS KKPWKHLWFV IKNKVLYTYA ASEDVAALES QPLLGFTVTL VKDENSESKV FQLLHKGMVF YVFKADDAHS TQRWIDAFQE GTVSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Pleckstrin Homology domain	[U-13C, U-15N]		1.22 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGQ, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of the Pleckstrin Homology Domain of Mouse Ethanol Decreased 4 Protein
Li, H., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. FYVE, RhoGEF and PH domain containing 6; ethanol decreased 4, : 1 : 181 entities Detail

Experiments performed 2 experiments Detail