Solution Structure of the RA Domain of Human Grb7 Protein
GSSGSSGRPH VVKVYSEDGA CRSVEVAAGA TARHVCEMLV QRAHALSDET WGLVECHPHL ALERGLEDHE SVVEVQAAWP VGGDSRFVFR KNFASGPSSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.7 % (993 of 1060) | 92.8 % (503 of 542) | 94.7 % (395 of 417) | 94.1 % (95 of 101) |
Backbone | 91.7 % (543 of 592) | 89.4 % (185 of 207) | 92.7 % (268 of 289) | 93.8 % (90 of 96) |
Sidechain | 96.1 % (535 of 557) | 94.9 % (318 of 335) | 97.7 % (212 of 217) | 100.0 % (5 of 5) |
Aromatic | 97.7 % (84 of 86) | 97.7 % (42 of 43) | 97.6 % (40 of 41) | 100.0 % (2 of 2) |
Methyl | 98.0 % (100 of 102) | 96.1 % (49 of 51) | 100.0 % (51 of 51) |
1. RA domain
GSSGSSGRPH VVKVYSEDGA CRSVEVAAGA TARHVCEMLV QRAHALSDET WGLVECHPHL ALERGLEDHE SVVEVQAAWP VGGDSRFVFR KNFASGPSSGPressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RA domain | [U-13C, U-15N] | 1.17 mM | |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | 0.02 % | ||
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |