BMRB: 10043

Solution Structure of the N-terminal Domain of Mouse Putative Signal Recoginition Particle 54 (SRP54)

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Signal Recoginition Particle 54

Entity

1. Signal Recoginition Particle 54 (polymer, Thiol state: all free), 99 monomers, 10601.17 Da Detail

GSSGSSGADL GRKITSALRS LSNATIINEE VLNAMLKEVC TALLEADVNI KLVKQLRENV KSAIDLEEMA SGLNKRKMIQ HAVFKELVKV KVYSGPSSG

Formula weight

10601.17 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 92.1 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (1031 of 1120)	90.2 % (525 of 582)	93.5 % (404 of 432)	96.2 % (102 of 106)
Backbone	94.3 % (558 of 592)	92.2 % (188 of 204)	95.2 % (276 of 290)	95.9 % (94 of 98)
Sidechain	90.8 % (563 of 620)	89.2 % (337 of 378)	93.2 % (218 of 234)	100.0 % (8 of 8)
Aromatic	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)
Methyl	93.9 % (124 of 132)	92.4 % (61 of 66)	95.5 % (63 of 66)

1. N-terminal domain

GSSGSSGADL GRKITSALRS LSNATIINEE VLNAMLKEVC TALLEADVNI KLVKQLRENV KSAIDLEEMA SGLNKRKMIQ HAVFKELVKV KVYSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Protein	[U-13C, U-15N]		1.04 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGW, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of the N-terminal Domain of Mouse Putative Signal Recoginition Particle 54 (SRP54)
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Signal Recoginition Particle 54, : 1 : 147 entities Detail

Interaction partners 1. Signal Recoginition Particle 54, : 4 interactors Detail

Experiments performed 2 experiments Detail