BMRB: 10044

Solution structure of homeobox domain of Arabidopsisthaliana zinc finger homeobox family protein

Authors

Kaneno, D., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ZF-HD homeobox family protein

Entity

1. ZF-HD homeobox family protein (polymer, Thiol state: all free), 80 monomers, 8887.834 Da Detail

GSSGSSGSSA EAGGGIRKRH RTKFTAEQKE RMLALAERIG WRIQRQDDEV IQRFCQETGV PRQVLKVWLH NNKHSGPSSG

Formula weight

8887.834 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 90.9 %, Completeness (bb): 88.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (832 of 915)	90.3 % (438 of 485)	91.8 % (314 of 342)	90.9 % (80 of 88)
Backbone	88.7 % (422 of 476)	86.9 % (146 of 168)	89.6 % (206 of 230)	89.7 % (70 of 78)
Sidechain	93.3 % (475 of 509)	92.1 % (292 of 317)	95.1 % (173 of 182)	100.0 % (10 of 10)
Aromatic	89.3 % (50 of 56)	89.3 % (25 of 28)	88.5 % (23 of 26)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. homeobox domain

GSSGSSGSSA EAGGGIRKRH RTKFTAEQKE RMLALAERIG WRIQRQDDEV IQRFCQETGV PRQVLKVWLH NNKHSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-15N, U-13C]		1.1 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WH5, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution structure of homeobox domain of Arabidopsisthaliana zinc finger homeobox family protein
Kaneno, D., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. ZF-HD homeobox family protein, : 1 : 50 entities Detail

Interaction partners 1. ZF-HD homeobox family protein, : 14 interactors Detail

Experiments performed 2 experiments Detail