BMRB: 10045

Solution structure of homeobox domain of Arabidopsis thaliana hypothetical protein F22K18.140

Authors

Kaneno, D., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ZF-HD homeobox family protein

Entity

1. ZF-HD homeobox family protein (polymer, Thiol state: all free), 80 monomers, 8786.697 Da Detail

GSSGSSGSNP SSSGGTTKRF RTKFTAEQKE KMLAFAERLG WRIQKHDDVA VEQFCAETGV RRQVLKIWMH NNKNSGPSSG

Formula weight

8786.697 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 95.1 %, Completeness (bb): 93.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (865 of 910)	94.8 % (454 of 479)	95.6 % (328 of 343)	94.3 % (83 of 88)
Backbone	93.1 % (443 of 476)	92.2 % (154 of 167)	93.5 % (216 of 231)	93.6 % (73 of 78)
Sidechain	97.2 % (491 of 505)	96.2 % (300 of 312)	98.9 % (181 of 183)	100.0 % (10 of 10)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (34 of 34)	100.0 % (2 of 2)
Methyl	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)

1. homeobox domain

GSSGSSGSNP SSSGGTTKRF RTKFTAEQKE KMLAFAERLG WRIQKHDDVA VEQFCAETGV RRQVLKIWMH NNKNSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]		0.9 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WH7, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of homeobox domain of Arabidopsis thaliana hypothetical protein F22K18.140
Kaneno, D., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. ZF-HD homeobox family protein, : 1 : 2 : 75 entities Detail

Interaction partners 1. ZF-HD homeobox family protein, : 11 interactors Detail

Experiments performed 2 experiments Detail