BMRB: 10046

Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)

Authors

Yoneyama, M., Hayashi, F., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Mitogen activated protein kinase kinase 5

Entity

1. Mitogen activated protein kinase kinase 5 (polymer, Thiol state: all free), 113 monomers, 12400.74 Da Detail

GSSGSSGPFC AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRSGP SSG

Formula weight

12400.74 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 94.1 %, Completeness (bb): 92.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.1 % (1197 of 1272)	93.8 % (617 of 658)	95.4 % (474 of 497)	90.6 % (106 of 117)
Backbone	92.0 % (611 of 664)	90.4 % (207 of 229)	93.6 % (308 of 329)	90.6 % (96 of 106)
Sidechain	96.1 % (683 of 711)	95.6 % (410 of 429)	97.0 % (263 of 271)	90.9 % (10 of 11)
Aromatic	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (43 of 43)	100.0 % (1 of 1)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. PB1 domain

GSSGSSGPFC AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRSGP SSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 297 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PB1 domain	[U-13C; U-15N]		1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WI0, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)
Yoneyama, M., Hayashi, F., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Mitogen activated protein kinase kinase 5, : 1 : 12 entities Detail

Interaction partners 1. Mitogen activated protein kinase kinase 5, : 11 interactors Detail

Experiments performed 2 experiments Detail