BMRB: 10047

Solution Structure of the Homeobox Domain of Human Homeodomain Leucine Zipper-Encoding Gene (Homez)

Authors

Kamatari, Y., Tochio, N., Nameki, N., Miyamoto, K., Li, H., Kobayashi, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox leucine zipper protein Homez

Entity

1. Homeobox leucine zipper protein Homez (polymer, Thiol state: all free), 76 monomers, 8715.744 Da Detail

GSSGSSGKRK TKEQLAILKS FFLQCQWARR EDYQKLEQIT GLPRPEIIQW FGDTRYALKH GQLKWFRDNA SGPSSG

Formula weight

8715.744 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

tortion angle dynamics, restrained simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 93.2 %, Completeness (bb): 89.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (864 of 927)	93.1 % (458 of 492)	94.6 % (332 of 351)	88.1 % (74 of 84)
Backbone	89.3 % (402 of 450)	87.9 % (138 of 157)	91.4 % (201 of 220)	86.3 % (63 of 73)
Sidechain	96.3 % (525 of 545)	95.5 % (320 of 335)	97.5 % (194 of 199)	100.0 % (11 of 11)
Aromatic	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (45 of 45)	100.0 % (3 of 3)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. homeobox domain

GSSGSSGKRK TKEQLAILKS FFLQCQWARR EDYQKLEQIT GLPRPEIIQW FGDTRYALKH GQLKWFRDNA SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]		1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.26 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECC, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of the Homeobox Domain of Human Homeodomain Leucine Zipper-Encoding Gene (Homez)
Kamatari, Y., Tochio, N., Nameki, N., Miyamoto, K., Li, H., Kobayashi, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox leucine zipper protein Homez, : 1 : 6 : 56 entities Detail

Interaction partners 1. Homeobox leucine zipper protein Homez, : 35 interactors Detail

Experiments performed 2 experiments Detail