BMRB: 10048

Solution Structure of the UBA Domain of Human Tudor Domain Containing Protein 3

Authors

Kamatari, Y., Tochio, N., Nakanishi, T., Miyamoto, K., Li, H., Kobayashi, N., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Tudor domain containing protein 3

Entity

1. Tudor domain containing protein 3 (polymer, Thiol state: all free), 63 monomers, 6429.103 Da Detail

GSSGSSGVDE KALKHITEMG FSKEASRQAL MDNGNNLEAA LNVLLTSNKQ KPVMGPPSGP SSG

Formula weight

6429.103 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.1 %, Completeness: 91.8 %, Completeness (bb): 88.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (617 of 672)	90.6 % (319 of 352)	94.1 % (239 of 254)	89.4 % (59 of 66)
Backbone	88.6 % (328 of 370)	84.6 % (110 of 130)	91.7 % (166 of 181)	88.1 % (52 of 59)
Sidechain	95.5 % (341 of 357)	94.1 % (209 of 222)	97.7 % (125 of 128)	100.0 % (7 of 7)
Aromatic	100.0 % (14 of 14)	100.0 % (7 of 7)	100.0 % (7 of 7)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. UBA domain

GSSGSSGVDE KALKHITEMG FSKEASRQAL MDNGNNLEAA LNVLLTSNKQ KPVMGPPSGP SSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	UBA domain	[U-13C; U-15N]		1.04 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WJI, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution Structure of the UBA Domain of Human Tudor Domain Containing Protein 3
Kamatari, Y., Tochio, N., Nakanishi, T., Miyamoto, K., Li, H., Kobayashi, N., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Tudor domain containing protein 3, : 1 : 38 entities Detail

Experiments performed 2 experiments Detail