BMRB: 10050

Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1

Authors

Yoneyama, M., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

NEDD8 ultimate buster-1

Entity

1. NEDD8 ultimate buster-1 (polymer, Thiol state: all free), 100 monomers, 11020.51 Da Detail

GSSGSSGDNY RTTGIATIEV FLPPRLKKDR KNLLETRLHI TGRELRSKIA ETFGLQENYI KIVINKKQLQ LGKTLEEQGV AHNVKAMVLE LKQSSGPSSG

Formula weight

11020.51 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 87.4 %, Completeness (bb): 87.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.4 % (1026 of 1174)	85.4 % (528 of 618)	89.8 % (403 of 449)	88.8 % (95 of 107)
Backbone	87.4 % (519 of 594)	85.0 % (176 of 207)	87.6 % (254 of 290)	91.8 % (89 of 97)
Sidechain	87.6 % (587 of 670)	85.6 % (352 of 411)	92.0 % (229 of 249)	60.0 % (6 of 10)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	94.9 % (112 of 118)	91.5 % (54 of 59)	98.3 % (58 of 59)

1. Ubiquitin-like domain

GSSGSSGDNY RTTGIATIEV FLPPRLKKDR KNLLETRLHI TGRELRSKIA ETFGLQENYI KIVINKKQLQ LGKTLEEQGV AHNVKAMVLE LKQSSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-like domain	[U-13C; U-15N]		1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJU, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1
Yoneyama, M., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. NEDD8 ultimate buster-1, : 1 : 10 entities Detail

Interaction partners 1. NEDD8 ultimate buster-1, : 7 interactors Detail

Experiments performed 2 experiments Detail