BMRB: 10054

Solution structure of the first cold-shock domain of the human KIAA0885 protein (UNR protein)

Authors

Goroncy, A., Kigawa, T., Koshiba, S., Tomizawa, T., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.

Assembly

UNR protein

Entity

1. UNR protein (polymer, Thiol state: all free), 89 monomers, 9458.406 Da Detail

GSSGSSGGYP NGTSAALRET GVIEKLLTSY GFIQCSERQA RLFFHCSQYN GNLQDLKVGD DVEFEVSSDR RTGKPIAVKL VKISGPSSG

Formula weight

9458.406 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.7 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.7 % (967 of 980)	98.2 % (500 of 509)	99.5 % (376 of 378)	97.8 % (91 of 93)
Backbone	98.3 % (519 of 528)	97.3 % (182 of 187)	99.2 % (253 of 255)	97.7 % (84 of 86)
Sidechain	99.2 % (525 of 529)	98.8 % (318 of 322)	100.0 % (200 of 200)	100.0 % (7 of 7)
Aromatic	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. First cold-shock domain

GSSGSSGGYP NGTSAALRET GVIEKLLTSY GFIQCSERQA RLFFHCSQYN GNLQDLKVGD DVEFEVSSDR RTGKPIAVKL VKISGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	cold-shock domain	[U-13C; U-15N]		1.09 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 6 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.51 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFQ, Strand ID: A Detail

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Release date

2008-08-12

Citation

The NMR solution structures of the five constituent cold-shock domains (CSD) of the human UNR (upstream of N-ras) protein
Goroncy, A., Kigawa, T., Koshiba, S., Tomizawa, T., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.
J. Struct. Funct. Genomics (2010), 11, 181-188, PubMed 20213426 , DOI 10.1007/s10969-010-9081-z , Abstract

Related entities 1. UNR protein, : 1 : 2 : 15 entities Detail

Interaction partners 1. UNR protein, : 50 interactors Detail

Experiments performed 2 experiments Detail