BMRB: 10055

Solution Structure of Glia Maturation Factor-gamma from Mus Musculus

Authors

Goroncy, A., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.

Assembly

Glia maturation factor gamma

Entity

1. Glia maturation factor gamma (polymer, Thiol state: all free), 151 monomers, 17158.31 Da Detail

GSSGSSGSDS LVVCEVDPEL KETLRKFRFR KETNNAAIIM KVDKDRQMVV LEDELQNISP EELKLELPER QPRFVVYSYK YVHDDGRVSY PLCFIFSSPV GCKPEQQMMY AGSKNRLVQT AELTKVFEIR TTDDLTETWL KEKLASGPSS G

Formula weight

17158.31 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.7 %, Completeness (bb): 99.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.7 % (1775 of 1780)	100.0 % (934 of 934)	99.3 % (687 of 692)	100.0 % (154 of 154)
Backbone	99.4 % (885 of 890)	100.0 % (302 of 302)	98.9 % (440 of 445)	100.0 % (143 of 143)
Sidechain	100.0 % (1033 of 1033)	100.0 % (632 of 632)	100.0 % (390 of 390)	100.0 % (11 of 11)
Aromatic	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (57 of 57)	100.0 % (1 of 1)
Methyl	100.0 % (154 of 154)	100.0 % (77 of 77)	100.0 % (77 of 77)

1. COFILIN-ADF-domain

GSSGSSGSDS LVVCEVDPEL KETLRKFRFR KETNNAAIIM KVDKDRQMVV LEDELQNISP EELKLELPER QPRFVVYSYK YVHDDGRVSY PLCFIFSSPV GCKPEQQMMY AGSKNRLVQT AELTKVFEIR TTDDLTETWL KEKLASGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	cofilin	[U-13C; U-15N]		1.21 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.25 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WFS, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution Structure of Glia Maturation Factor-gamma from Mus Musculus
Goroncy, A., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.

Related entities 1. Glia maturation factor gamma, : 1 : 4 : 50 entities Detail

Interaction partners 1. Glia maturation factor gamma, : 2 interactors Detail

Experiments performed 2 experiments Detail