BMRB: 10056

Solution structure of the Ras-binding domain of mouse RGS14

Authors

Nakanishi, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

regulator of G-protein signaling 14; rap1/rap2 interacting protein

Entity

1. regulator of G-protein signaling 14; rap1/rap2 interacting protein (polymer, Thiol state: all free), 104 monomers, 11155.47 Da Detail

GSSGSSGDQE VRLENRITFQ LELVGLERVV RISAKPTKRL QEALQPILAK HGLSLDQVVL HRPGEKQPMD LENPVSSVAS QTLVLDTPPD AKMSEARSSG PSSG

Formula weight

11155.47 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 92.0 %, Completeness (bb): 89.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (1070 of 1163)	91.3 % (558 of 611)	94.2 % (421 of 447)	86.7 % (91 of 105)
Backbone	89.8 % (546 of 608)	88.0 % (183 of 208)	92.1 % (280 of 304)	86.5 % (83 of 96)
Sidechain	94.5 % (615 of 651)	93.1 % (375 of 403)	97.1 % (232 of 239)	88.9 % (8 of 9)
Aromatic	77.8 % (14 of 18)	77.8 % (7 of 9)	77.8 % (7 of 9)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. Raf-like Ras-binding domain

GSSGSSGDQE VRLENRITFQ LELVGLERVV RISAKPTKRL QEALQPILAK HGLSLDQVVL HRPGEKQPMD LENPVSSVAS QTLVLDTPPD AKMSEARSSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RBD	[U-13C; U-15N]		0.18 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WFY, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution structure of the Ras-binding domain of mouse RGS14
Nakanishi, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. regulator of G-protein signaling 14; rap1/rap2 interacting protein, : 1 : 2 : 15 entities Detail

Interaction partners 1. regulator of G-protein signaling 14; rap1/rap2 interacting protein, : 3 interactors Detail

Experiments performed 2 experiments Detail