BMRB: 10057

Solution structure of Iron-sulfur cluster protein U (IscU)

Authors

Nakanishi, T., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

nitrogen fixation cluster-like

Entity

1. Iron-sulfur cluster protein U (IscU) (polymer, Thiol state: all free), 130 monomers, 13426.17 Da Detail

GSSGSSGENP RNVGSLDKTS KNVGTGLVGA PACGDVMKLQ IQVDEKGKIV DARFKTFGCG SAIASSSLAT EWVKGKTVEE ALTIKNTDIA KELCLPPVKL HCSMLAEDAI KAALADYKLK QESKSGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

13491.579 Da

Max. entity weight

13426.17 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS33:SG	2:ZN1:ZN
2	na	sing	1:ASP35:OD2	2:ZN1:ZN
3	na	sing	1:CYS59:SG	2:ZN1:ZN
4	na	sing	1:HIS101:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 95.3 %, Completeness (bb): 92.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (1345 of 1412)	96.4 % (706 of 732)	94.0 % (514 of 547)	94.0 % (125 of 133)
Backbone	92.5 % (712 of 770)	93.7 % (252 of 269)	91.2 % (343 of 376)	93.6 % (117 of 125)
Sidechain	98.5 % (747 of 758)	98.1 % (454 of 463)	99.3 % (285 of 287)	100.0 % (8 of 8)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (21 of 21)	100.0 % (1 of 1)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. Iron-sulfur cluster protein U (IscU)

GSSGSSGENP RNVGSLDKTS KNVGTGLVGA PACGDVMKLQ IQVDEKGKIV DARFKTFGCG SAIASSSLAT EWVKGKTVEE ALTIKNTDIA KELCLPPVKL HCSMLAEDAI KAALADYKLK QESKSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	IscU domain	[U-13C; U-15N]		1.12 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	10 mM
5	NaN3			0.02 %
6	ZnCl2			0.01 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFZ, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of Iron-sulfur cluster protein U (IscU)
Nakanishi, T., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Iron-sulfur cluster protein U (IscU), : 1 : 11 : 73 entities Detail

Interaction partners 1. Iron-sulfur cluster protein U (IscU), : 1 interactors Detail

Experiments performed 2 experiments Detail