BMRB: 10058

Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein

Authors

Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Thioredoxin-like protein 2

Entity

1. Thioredoxin-like protein 2 (polymer, Thiol state: all free), 109 monomers, 11828.29 Da Detail

GSSGSSGLKV LTNKASVMLF MKGNKQEAKC GFSKQILEIL NSTGVEYETF DILEDEEVRQ GLKTFSNWPT YPQLYVRGDL VGGLDIVKEL KDNGELLPIL KGESGPSSG

Formula weight

11828.29 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 91.8 %, Completeness (bb): 91.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (1154 of 1257)	89.0 % (584 of 656)	95.3 % (463 of 486)	93.0 % (107 of 115)
Backbone	91.8 % (593 of 646)	87.7 % (200 of 228)	94.2 % (295 of 313)	93.3 % (98 of 105)
Sidechain	92.6 % (654 of 706)	89.7 % (384 of 428)	97.4 % (261 of 268)	90.0 % (9 of 10)
Aromatic	89.5 % (68 of 76)	81.6 % (31 of 38)	97.3 % (36 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. PICOT homology 2 domain

GSSGSSGLKV LTNKASVMLF MKGNKQEAKC GFSKQILEIL NSTGVEYETF DILEDEEVRQ GLKTFSNWPT YPQLYVRGDL VGGLDIVKEL KDNGELLPIL KGESGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PICOT homology 2 domain	[U-13C; U-15N]		0.65 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.32 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.32 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WIK, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Thioredoxin-like protein 2, : 1 : 176 entities Detail

Interaction partners 1. Thioredoxin-like protein 2, : 1 interactors Detail

Experiments performed 2 experiments Detail