BMRB: 10059

Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein

Authors

Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Flotillin 2

Entity

1. Flotillin 2 (polymer, Thiol state: all free), 143 monomers, 15460.46 Da Detail

GSSGSSGQRI SLEIMTLQPR CEDVETAEGV ALTVTGVAQV KIMTEKELLA VACEQFLGKN VQDIKNVVLQ TLEGHLRSIL GTLTVEQIYQ DRDQFAKLVR EVAAPDVGRM GIEILSFTIK DVYDKVDYLS SLGKTQTSGP SSG

Formula weight

15460.46 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 96.5 %, Completeness (bb): 95.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1552 of 1608)	95.7 % (793 of 829)	97.6 % (612 of 627)	96.7 % (147 of 152)
Backbone	95.5 % (814 of 852)	93.2 % (276 of 296)	96.9 % (403 of 416)	96.4 % (135 of 140)
Sidechain	97.9 % (867 of 886)	97.0 % (517 of 533)	99.1 % (338 of 341)	100.0 % (12 of 12)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)
Methyl	100.0 % (194 of 194)	100.0 % (97 of 97)	100.0 % (97 of 97)

1. Band 7 Domain

GSSGSSGQRI SLEIMTLQPR CEDVETAEGV ALTVTGVAQV KIMTEKELLA VACEQFLGKN VQDIKNVVLQ TLEGHLRSIL GTLTVEQIYQ DRDQFAKLVR EVAAPDVGRM GIEILSFTIK DVYDKVDYLS SLGKTQTSGP SSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Band 7 Domain	[U-13C; U-15N]		1.02 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WIN, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein
Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Flotillin 2, : 1 : 34 entities Detail

Interaction partners 1. Flotillin 2, : 2 interactors Detail

Experiments performed 2 experiments Detail