BMRB: 10060

Solution structure of hypothetical protein C330018D20Rik from Mus musculus

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

C330018D20rik protein

Entity

1. C330018D20rik protein (polymer, Thiol state: all free), 100 monomers, 11244.74 Da Detail

GSSGSSGNLS ASNRALPVLT LFTKAPCPLC DEAKEVLQPY KDRFILQEVD ITLPENSTWY ERYKFDIPVF HLNGQFLMMH RVNTSKLEKQ LRKLSGPSSG

Formula weight

11244.74 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 94.1 %, Completeness (bb): 92.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.1 % (1117 of 1187)	94.4 % (587 of 622)	94.4 % (436 of 462)	91.3 % (94 of 103)
Backbone	92.5 % (542 of 586)	92.5 % (184 of 199)	92.5 % (272 of 294)	92.5 % (86 of 93)
Sidechain	96.0 % (667 of 695)	95.3 % (403 of 423)	97.7 % (256 of 262)	80.0 % (8 of 10)
Aromatic	94.7 % (89 of 94)	95.7 % (45 of 47)	93.5 % (43 of 46)	100.0 % (1 of 1)
Methyl	99.0 % (101 of 102)	98.0 % (50 of 51)	100.0 % (51 of 51)

1. glutaredoxin domain

GSSGSSGNLS ASNRALPVLT LFTKAPCPLC DEAKEVLQPY KDRFILQEVD ITLPENSTWY ERYKFDIPVF HLNGQFLMMH RVNTSKLEKQ LRKLSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	glutaredoxin domain	[U-13C; U-15N]		0.6 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	10 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJK, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of hypothetical protein C330018D20Rik from Mus musculus
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C330018D20rik protein, : 1 : 2 : 16 entities Detail

Experiments performed 2 experiments Detail