BMRB: 10061

Solution structure of PDZ domain of mouse Cypher protein

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Cypher protein

Entity

1. Cypher protein (polymer, Thiol state: all free), 90 monomers, 9390.432 Da Detail

GSSGSSGMSY SVTLTGPGPW GFRLQGGKDF NMPLTISRIT PGSKAAQSQL SQGDLVVAID GVNTDTMTHL EAQNKIKSAS YNLSLTLQKS

Formula weight

9390.432 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 98.5 %, Completeness (bb): 98.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.5 % (969 of 984)	98.6 % (501 of 508)	98.4 % (373 of 379)	97.9 % (95 of 97)
Backbone	98.1 % (522 of 532)	98.4 % (184 of 187)	98.1 % (254 of 259)	97.7 % (84 of 86)
Sidechain	98.9 % (525 of 531)	98.8 % (317 of 321)	99.0 % (197 of 199)	100.0 % (11 of 11)
Aromatic	96.2 % (50 of 52)	96.2 % (25 of 26)	96.0 % (24 of 25)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. PDZ domain

GSSGSSGMSY SVTLTGPGPW GFRLQGGKDF NMPLTISRIT PGSKAAQSQL SQGDLVVAID GVNTDTMTHL EAQNKIKSAS YNLSLTLQKS

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.1 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WJL, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution structure of PDZ domain of mouse Cypher protein
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Cypher protein, : 1 : 4 : 151 entities Detail

Interaction partners 1. Cypher protein, : 1 interactors Detail

Experiments performed 2 experiments Detail