Solution structure of PDZ domain of mouse Cypher protein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 98.5 % (969 of 984) | 98.6 % (501 of 508) | 98.4 % (373 of 379) | 97.9 % (95 of 97) |
Backbone | 98.1 % (522 of 532) | 98.4 % (184 of 187) | 98.1 % (254 of 259) | 97.7 % (84 of 86) |
Sidechain | 98.9 % (525 of 531) | 98.8 % (317 of 321) | 99.0 % (197 of 199) | 100.0 % (11 of 11) |
Aromatic | 96.2 % (50 of 52) | 96.2 % (25 of 26) | 96.0 % (24 of 25) | 100.0 % (1 of 1) |
Methyl | 100.0 % (94 of 94) | 100.0 % (47 of 47) | 100.0 % (47 of 47) |
1. PDZ domain
GSSGSSGMSY SVTLTGPGPW GFRLQGGKDF NMPLTISRIT PGSKAAQSQL SQGDLVVAID GVNTDTMTHL EAQNKIKSAS YNLSLTLQKSPressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PDZ domain | [U-13C; U-15N] | 1.1 mM | |
2 | phosphate | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | 0.02 % | ||
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |