BMRB: 10063

Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)

Authors

Tochio, N., Kigawa, T., Koshiba, S., Kobayashi, N., Inoue, M., Yokoyama, S.

Assembly

Intersectin 2

Entity

1. Intersectin 2 (polymer, Thiol state: all free), 80 monomers, 8282.172 Da Detail

GSSGSSGEIA QVTSAYVASG SEQLSLAPGQ LILILKKNTS GWWQGELQAR GKKRQKGWFP ASHVKLLGPS SERASGPSSG

Formula weight

8282.172 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 89.2 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.2 % (790 of 886)	88.8 % (411 of 463)	90.2 % (304 of 337)	87.2 % (75 of 86)
Backbone	86.7 % (409 of 472)	86.9 % (146 of 168)	86.8 % (198 of 228)	85.5 % (65 of 76)
Sidechain	91.7 % (442 of 482)	89.8 % (265 of 295)	94.4 % (167 of 177)	100.0 % (10 of 10)
Aromatic	94.8 % (55 of 58)	89.7 % (26 of 29)	100.0 % (26 of 26)	100.0 % (3 of 3)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. SH3 domain

GSSGSSGEIA QVTSAYVASG SEQLSLAPGQ LILILKKNTS GWWQGELQAR GKKRQKGWFP ASHVKLLGPS SERASGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]		1.4 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.4 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UE9, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)
Tochio, N., Kigawa, T., Koshiba, S., Kobayashi, N., Inoue, M., Yokoyama, S.

Related entities 1. Intersectin 2, : 1 : 139 entities Detail

Interaction partners 1. Intersectin 2, : 34 interactors Detail

Experiments performed 2 experiments Detail