BMRB: 10064

Solution Structure of The forth PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2)

Entity

1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2) (polymer, Thiol state: all free), 114 monomers, 11899.37 Da Detail

GSSGSSGSLQ TSDVVIHRKE NEGFGFVIIS SLNRPESGST ITVPHKIGRI IDGSPADRCA KLKVGDRILA VNGQSIINMP HADIVKLIKD AGLSVTLRII PQEELNSPSG PSSG

Formula weight

11899.37 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 94.7 %, Completeness (bb): 93.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1184 of 1250)	94.0 % (611 of 650)	95.9 % (465 of 485)	93.9 % (108 of 115)
Backbone	93.1 % (624 of 670)	91.9 % (215 of 234)	93.9 % (309 of 329)	93.5 % (100 of 107)
Sidechain	96.5 % (657 of 681)	95.2 % (396 of 416)	98.4 % (253 of 257)	100.0 % (8 of 8)
Aromatic	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	98.6 % (136 of 138)	97.1 % (67 of 69)	100.0 % (69 of 69)

1. PDZ Domain

GSSGSSGSLQ TSDVVIHRKE NEGFGFVIIS SLNRPESGST ITVPHKIGRI IDGSPADRCA KLKVGDRILA VNGQSIINMP HADIVKLIKD AGLSVTLRII PQEELNSPSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.4 mM
2	Tris-HCl(7.0)		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UEW, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution Structure of The forth PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2), : 1 : 155 entities Detail

Interaction partners 1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2), : 8 interactors Detail

Experiments performed 2 experiments Detail