BMRB: 10068

Solution Structure of the CH domain from Mouse Trangelin

Authors

Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

Transgelin

Entity

1. Transgelin (polymer, Thiol state: all free), 144 monomers, 15785.79 Da Detail

GSSGSSGEEL EERLVEWIVV QCGPDVGRPD RGRLGFQVWL KNGVILSKLV NSLYPEGSKP VKVPENPPSM VFKQMEQVAQ FLKAAEDYGV IKTDMFQTVD LYEGKDMAAV QRTLMALGSL AVTKNDGNYR GDPNWFMKSG PSSG

Formula weight

15785.79 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 95.3 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (1580 of 1658)	95.3 % (826 of 867)	95.6 % (612 of 640)	94.0 % (142 of 151)
Backbone	93.7 % (793 of 846)	93.9 % (277 of 295)	93.8 % (390 of 416)	93.3 % (126 of 135)
Sidechain	97.0 % (912 of 940)	96.0 % (549 of 572)	98.6 % (347 of 352)	100.0 % (16 of 16)
Aromatic	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (56 of 56)	100.0 % (3 of 3)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. Calponin homology (CH) domain

GSSGSSGEEL EERLVEWIVV QCGPDVGRPD RGRLGFQVWL KNGVILSKLV NSLYPEGSKP VKVPENPPSM VFKQMEQVAQ FLKAAEDYGV IKTDMFQTVD LYEGKDMAAV QRTLMALGSL AVTKNDGNYR GDPNWFMKSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]		1.0 mM
2	Tris-HCl(pH 7.0)		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJO, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution Structure of the CH domain from Mouse Trangelin
Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. Transgelin, : 1 : 2 : 61 entities Detail

Interaction partners 1. Transgelin, : 6 interactors Detail

Experiments performed 2 experiments Detail