BMRB: 10070

Solution structure of RRM domain in heterogeneous nuclear ribonucleoprotein H'

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Heterogeneous nuclear ribonucleoprotein H'

Entity

1. Heterogeneous nuclear ribonucleoprotein H' (polymer, Thiol state: all free), 102 monomers, 10807.71 Da Detail

GSSGSSGSSF QSTTGHCVHM RGLPYRATEN DIYNFFSPLN PMRVHIEIGP DGRVTGEADV EFATHEDAVA AMAKDKANMQ HRYVELFLNS TAGTSGSGPS SG

Formula weight

10807.71 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 93.3 %, Completeness (bb): 91.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (1010 of 1082)	92.8 % (515 of 555)	94.6 % (400 of 423)	91.3 % (95 of 104)
Backbone	91.9 % (553 of 602)	90.5 % (191 of 211)	93.2 % (274 of 294)	90.7 % (88 of 97)
Sidechain	94.9 % (541 of 570)	94.2 % (324 of 344)	95.9 % (210 of 219)	100.0 % (7 of 7)
Aromatic	95.7 % (90 of 94)	95.7 % (45 of 47)	95.7 % (45 of 47)
Methyl	97.6 % (82 of 84)	97.6 % (41 of 42)	97.6 % (41 of 42)

1. RRM domain

GSSGSSGSSF QSTTGHCVHM RGLPYRATEN DIYNFFSPLN PMRVHIEIGP DGRVTGEADV EFATHEDAVA AMAKDKANMQ HRYVELFLNS TAGTSGSGPS SG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]		0.8 mM
2	d-Tris-HCl(pH 7.0)		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WEZ, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of RRM domain in heterogeneous nuclear ribonucleoprotein H'
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. Heterogeneous nuclear ribonucleoprotein H', : 1 : 7 : 79 entities Detail

Interaction partners 1. Heterogeneous nuclear ribonucleoprotein H', : 67 interactors Detail

Experiments performed 2 experiments Detail