BMRB: 10071

Solution structure of RRM domain in RNA-binding protein NP_057951

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

RNA-binding protein Raly

Entity

1. RNA-binding protein Raly (polymer, Thiol state: all free), 110 monomers, 11556.80 Da Detail

GSSGSSGMSL KLQASNVTNK NDPKSINSRV FIGNLNTALV KKSDVETIFS KYGRVAGCSV HKGYAFVQYS NERHARAAVL GENGRVLAGQ TLDINMAGEP KPDRSGPSSG

Formula weight

11556.8 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 95.0 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.0 % (1141 of 1201)	94.6 % (591 of 625)	96.5 % (442 of 458)	91.5 % (108 of 118)
Backbone	94.2 % (614 of 652)	93.4 % (214 of 229)	95.0 % (301 of 317)	93.4 % (99 of 106)
Sidechain	96.0 % (620 of 646)	95.2 % (377 of 396)	98.3 % (234 of 238)	75.0 % (9 of 12)
Aromatic	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. RRM domain

GSSGSSGMSL KLQASNVTNK NDPKSINSRV FIGNLNTALV KKSDVETIFS KYGRVAGCSV HKGYAFVQYS NERHARAAVL GENGRVLAGQ TLDINMAGEP KPDRSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]		0.8 mM
2	d-Tris-HCl(pH 7.0)		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.37 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WF1, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of RRM domain in RNA-binding protein NP_057951
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. RNA-binding protein Raly, : 1 : 2 : 81 entities Detail

Interaction partners 1. RNA-binding protein Raly, : 58 interactors Detail

Experiments performed 2 experiments Detail