BMRB: 10072

Solution structure of RRM domain in HNRPC protein

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Heterogeneous nuclear ribonucleoproteins C1/C2

Entity

1. Heterogeneous nuclear ribonucleoproteins C1/C2 (polymer, Thiol state: all free), 98 monomers, 10273.49 Da Detail

GSSGSSGKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRSGPSSG

Formula weight

10273.49 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.8 %, Completeness: 90.5 %, Completeness (bb): 87.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.5 % (970 of 1072)	89.7 % (499 of 556)	92.5 % (381 of 412)	86.5 % (90 of 104)
Backbone	87.5 % (509 of 582)	85.4 % (175 of 205)	89.7 % (253 of 282)	85.3 % (81 of 95)
Sidechain	93.9 % (541 of 576)	92.6 % (325 of 351)	95.8 % (207 of 216)	100.0 % (9 of 9)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. RRM domain

GSSGSSGKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]		0.8 mM
2	d-Tris-HCl(pH 7.0)		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.34 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WF2, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Solution structure of RRM domain in HNRPC protein
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. Heterogeneous nuclear ribonucleoproteins C1/C2, : 1 : 148 entities Detail

Interaction partners 1. Heterogeneous nuclear ribonucleoproteins C1/C2, : 2 interactors Detail

Experiments performed 2 experiments Detail