BMRB: 10073

C2 domain-containing protein from putative elicitor-responsive gene

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

putative elicitor-responsive gene

Entity

1. putative elicitor-responsive gene (polymer, Thiol state: all free), 136 monomers, 14504.82 Da Detail

GSSGSSGPHG TLEVVLVSAK GLEDADFLNN MDPYVQLTCR TQDQKSNVAE GMGTTPEWNE TFIFTVSEGT TELKAKIFDK DVGTEDDAVG EATIPLEPVF VEGSIPPTAY NVVKDEEYKG EIWVALSFKP SGPSSG

Formula weight

14504.82 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 98.5 %, Completeness (bb): 96.4 % Detail

Download

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• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.5 % (1471 of 1493)	98.6 % (755 of 766)	99.0 % (584 of 590)	96.4 % (132 of 137)
Backbone	96.4 % (769 of 798)	95.3 % (264 of 277)	97.2 % (383 of 394)	96.1 % (122 of 127)
Sidechain	99.1 % (810 of 817)	99.0 % (484 of 489)	99.4 % (316 of 318)	100.0 % (10 of 10)
Aromatic	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (54 of 54)	100.0 % (2 of 2)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. C2 domain

GSSGSSGPHG TLEVVLVSAK GLEDADFLNN MDPYVQLTCR TQDQKSNVAE GMGTTPEWNE TFIFTVSEGT TELKAKIFDK DVGTEDDAVG EATIPLEPVF VEGSIPPTAY NVVKDEEYKG EIWVALSFKP SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	C2 domain	[U-13C; U-15N]		1.19 mM
2	NaPi			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.72 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFJ, Strand ID: A Detail

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Release date

2008-08-13

Citation

C2 domain-containing protein from putative elicitor-responsive gene
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. putative elicitor-responsive gene, : 1 : 2 : 80 entities Detail

Experiments performed 2 experiments Detail