BMRB: 10074

The first C2 domain of human synaptotagmin XIII

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

synaptotagmin XIII

Entity

1. synaptotagmin XIII (polymer, Thiol state: all free), 138 monomers, 14738.21 Da Detail

GSSGSSGSWN QAPKLHYCLD YDCQKAELFV TRLEAVTSNH DGGCDCYVQG SVANRTGSVE AQTALKKRQL HTTWEEGLVL PLAEEELPTA TLTLTLRTCD RFSRHSVAGE LRLGLDGTSV PLGAAQWGEL KTSGPSSG

Formula weight

14738.21 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 94.0 %, Completeness (bb): 92.2 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.0 % (1403 of 1493)	93.3 % (715 of 766)	95.7 % (557 of 582)	90.3 % (131 of 145)
Backbone	92.2 % (754 of 818)	90.6 % (259 of 286)	93.7 % (374 of 399)	91.0 % (121 of 133)
Sidechain	96.1 % (767 of 798)	95.0 % (456 of 480)	98.4 % (301 of 306)	83.3 % (10 of 12)
Aromatic	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (45 of 45)	100.0 % (3 of 3)
Methyl	100.0 % (152 of 152)	100.0 % (76 of 76)	100.0 % (76 of 76)

1. C2 domain

GSSGSSGSWN QAPKLHYCLD YDCQKAELFV TRLEAVTSNH DGGCDCYVQG SVANRTGSVE AQTALKKRQL HTTWEEGLVL PLAEEELPTA TLTLTLRTCD RFSRHSVAGE LRLGLDGTSV PLGAAQWGEL KTSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	C2 domain	[U-13C; U-15N]		1.07 mM
2	d-TRIS		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	5 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFM, Strand ID: A Detail

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Release date

2008-08-13

Citation

The first C2 domain of human synaptotagmin XIII
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. synaptotagmin XIII, : 1 : 129 entities Detail

Experiments performed 2 experiments Detail