BMRB: 10075

The fourth FN3 domain of human sidekick-2

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

sidekick 2

Entity

1. sidekick 2 (polymer, Thiol state: all free), 119 monomers, 12624.79 Da Detail

GSSGSSGPQL VRTHEDVPGP VGHLSFSEIL DTSLKVSWQE PGEKNGILTG YRISWEEYNR TNTRVTHYLP NVTLEYRVTG LTALTTYTIE VAAMTSKGQG QVSASTISSG VPPSGPSSG

Formula weight

12624.79 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 93.5 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.5 % (1209 of 1293)	93.2 % (618 of 663)	94.1 % (479 of 509)	92.6 % (112 of 121)
Backbone	91.5 % (639 of 698)	90.2 % (220 of 244)	92.4 % (317 of 343)	91.9 % (102 of 111)
Sidechain	95.6 % (669 of 700)	95.0 % (398 of 419)	96.3 % (261 of 271)	100.0 % (10 of 10)
Aromatic	95.3 % (82 of 86)	95.3 % (41 of 43)	95.1 % (39 of 41)	100.0 % (2 of 2)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. FN3 domain

GSSGSSGPQL VRTHEDVPGP VGHLSFSEIL DTSLKVSWQE PGEKNGILTG YRISWEEYNR TNTRVTHYLP NVTLEYRVTG LTALTTYTIE VAAMTSKGQG QVSASTISSG VPPSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]		1.21 mM
2	d-TRIS		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.85 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFN, Strand ID: A Detail

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Release date

2008-08-13

Citation

The fourth FN3 domain of human sidekick-2
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. sidekick 2, : 1 : 2 : 387 entities Detail

Interaction partners 1. sidekick 2, : 1 interactors Detail

Experiments performed 2 experiments Detail