BMRB: 10076

The eighth FN3 domain of human sidekick-2

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

sidekick 2

Entity

1. sidekick 2 (polymer, Thiol state: all free), 130 monomers, 13722.32 Da Detail

GSSGSSGRIG DGSPSHPPIL ERTLDDVPGP PMGILFPEVR TTSVRLIWQP PAAPNGIILA YQITHRLNTT TANTATVEVL APSARQYTAT GLKPESVYLF RITAQTRKGW GEAAEALVVT TEKRSGPSSG

Formula weight

13722.32 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 95.6 %, Completeness (bb): 94.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (1363 of 1426)	94.7 % (697 of 736)	97.0 % (547 of 564)	94.4 % (119 of 126)
Backbone	94.8 % (715 of 754)	93.5 % (243 of 260)	96.0 % (362 of 377)	94.0 % (110 of 117)
Sidechain	96.6 % (762 of 789)	95.4 % (454 of 476)	98.4 % (299 of 304)	100.0 % (9 of 9)
Aromatic	96.1 % (73 of 76)	97.4 % (37 of 38)	94.4 % (34 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (158 of 158)	100.0 % (79 of 79)	100.0 % (79 of 79)

1. FN3 domain

GSSGSSGRIG DGSPSHPPIL ERTLDDVPGP PMGILFPEVR TTSVRLIWQP PAAPNGIILA YQITHRLNTT TANTATVEVL APSARQYTAT GLKPESVYLF RITAQTRKGW GEAAEALVVT TEKRSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]		1.00 mM
2	d-TRIS		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WFO, Strand ID: A Detail

---

Release date

2008-08-13

Citation

The eighth FN3 domain of human sidekick-2
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. sidekick 2, : 1 : 5 : 357 entities Detail

Interaction partners 1. sidekick 2, : 1 interactors Detail

Experiments performed 2 experiments Detail