BMRB: 10077

Solution structure of the RNA binding domain of eukaryotic initiation factor 4B

Authors

Suzuki, S., Muto, Y., Nagata, T., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

Eukaryotic translation initiation factor 4B

Entity

1. Eukaryotic translation initiation factor 4B (polymer, Thiol state: all free), 104 monomers, 11235.21 Da Detail

GSSGSSGSRL PKSPPYTAFL GNLPYDVTEE SIKEFFRGLN ISAVRLPREP SNPERLKGFG YAEFEDLDSL LSALSLNEES LGNKRIRVDV ADQAQDKDSG PSSG

Formula weight

11235.21 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 93.0 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.0 % (1083 of 1164)	92.1 % (563 of 611)	94.0 % (423 of 450)	94.2 % (97 of 103)
Backbone	94.2 % (573 of 608)	92.4 % (194 of 210)	95.4 % (288 of 302)	94.8 % (91 of 96)
Sidechain	92.6 % (602 of 650)	92.0 % (369 of 401)	93.8 % (227 of 242)	85.7 % (6 of 7)
Aromatic	70.3 % (52 of 74)	70.3 % (26 of 37)	70.3 % (26 of 37)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. RNA recognition motif

GSSGSSGSRL PKSPPYTAFL GNLPYDVTEE SIKEFFRGLN ISAVRLPREP SNPERLKGFG YAEFEDLDSL LSALSLNEES LGNKRIRVDV ADQAQDKDSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RNA recognition motif	[U-13C; U-15N]		1.0 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.45 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WI8, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the RNA binding domain of eukaryotic initiation factor 4B
Suzuki, S., Muto, Y., Nagata, T., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Related entities 1. Eukaryotic translation initiation factor 4B, : 1 : 108 entities Detail

Interaction partners 1. Eukaryotic translation initiation factor 4B, : 53 interactors Detail

Experiments performed 2 experiments Detail