BMRB: 10079

Solution structure of putative domain of human KIAA0561 protein

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA0561 protein

Entity

1. KIAA0561 protein (polymer, Thiol state: all free), 114 monomers, 12409.98 Da Detail

GSSGSSGPKA TAQMEGRLQE FLTAYAPGAR LALADGVLGF IHHQIVELAR DCLAKSGENL VTSRYFLEMQ EKLERLLQDA HERSDSEEVS FIVQLVRKLL IIISRPARSG PSSG

Formula weight

12409.98 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 88.6 %, Completeness (bb): 88.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.6 % (1143 of 1290)	87.2 % (585 of 671)	90.8 % (456 of 502)	87.2 % (102 of 117)
Backbone	88.0 % (595 of 676)	86.3 % (202 of 234)	89.8 % (298 of 332)	86.4 % (95 of 110)
Sidechain	89.8 % (645 of 718)	87.6 % (383 of 437)	93.1 % (255 of 274)	100.0 % (7 of 7)
Aromatic	94.1 % (64 of 68)	94.1 % (32 of 34)	94.1 % (32 of 34)
Methyl	97.1 % (132 of 136)	97.1 % (66 of 68)	97.1 % (66 of 68)

1. putative domain (residues 180-280)

GSSGSSGPKA TAQMEGRLQE FLTAYAPGAR LALADGVLGF IHHQIVELAR DCLAKSGENL VTSRYFLEMQ EKLERLLQDA HERSDSEEVS FIVQLVRKLL IIISRPARSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	putative domain	[U-13C; U-15N]		1.3 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1V9V, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Solution structure of putative domain of human KIAA0561 protein
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA0561 protein, : 1 : 2 : 33 entities Detail

Interaction partners 1. KIAA0561 protein, : 8 interactors Detail

Experiments performed 2 experiments Detail