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Solution structure of mouse putative 42-9-9 protein
Authors
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Assembly
putative 42-9-9 protein
Entity
1. putative 42-9-9 protein (polymer, Thiol state: all free), 130 monomers, 14618.30 Da Detail

GSEGAATMAT FEEVSVLGFE EFDKAVKEHE SKTIFAYFSG SKDTEGKSWC PDCVEAEPVI REGLKHVTED CVFIYCQVGD KPYWKDPNND FRQKLKITAV PTLLKYGTPQ KLVESECCQS SLVEMIFSED


Formula weight
14618.3 Da
Source organism
Mus musculus
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 97.1 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.1 % (1465 of 1508)95.7 % (748 of 782)98.8 % (587 of 594)98.5 % (130 of 132)
Backbone98.3 % (755 of 768)97.3 % (255 of 262)99.0 % (378 of 382)98.4 % (122 of 124)
Sidechain96.5 % (832 of 862)94.8 % (493 of 520)99.1 % (331 of 334)100.0 % (8 of 8)
Aromatic97.9 % (141 of 144)97.2 % (70 of 72)98.6 % (69 of 70)100.0 % (2 of 2)
Methyl99.2 % (121 of 122)98.4 % (60 of 61)100.0 % (61 of 61)

1. putative 42-9-9 protein

GSEGAATMAT FEEVSVLGFE EFDKAVKEHE SKTIFAYFSG SKDTEGKSWC PDCVEAEPVI REGLKHVTED CVFIYCQVGD KPYWKDPNND FRQKLKITAV PTLLKYGTPQ KLVESECCQS SLVEMIFSED

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
1putative 42-9-9 protein[U-13C; U-15N]0.9 mM
2phosphatebuffer20 mM
3NaClsalt100 mM
4d-DTTsalt1 mM
5NaN30.02 %
6H2Osolvent90 %
7D2Osolvent10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 1V9W, Strand ID: A Detail


Release date
2008-08-13
Citation
Solution structure of mouse putative 42-9-9 protein
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Related entities 1. putative 42-9-9 protein, : 1 : 2 : 18 entities Detail
Interaction partners 1. putative 42-9-9 protein, : 3 interactors Detail
Experiments performed 2 experiments Detail
null