BMRB: 10082

Solution structure of RRM domain in calcipressin 1

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

calcipressin 1

Entity

1. calcipressin 1 (polymer, Thiol state: all free), 104 monomers, 11510.74 Da Detail

GSSGSSGLIA CVANDDVFSE SETRAKFESL FRTYDKDTTF QYFKSFKRVR INFSNPLSAA DARLRLHKTE FLGKEMKLYF AQTLHIGSSH LAPPNPDKSG PSSG

Formula weight

11510.74 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 94.7 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1137 of 1201)	93.9 % (587 of 625)	96.6 % (455 of 471)	90.5 % (95 of 105)
Backbone	93.2 % (572 of 614)	91.0 % (191 of 210)	95.7 % (292 of 305)	89.9 % (89 of 99)
Sidechain	96.5 % (660 of 684)	95.4 % (396 of 415)	98.1 % (258 of 263)	100.0 % (6 of 6)
Aromatic	95.2 % (120 of 126)	95.2 % (60 of 63)	95.2 % (60 of 63)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. RRM domain

GSSGSSGLIA CVANDDVFSE SETRAKFESL FRTYDKDTTF QYFKSFKRVR INFSNPLSAA DARLRLHKTE FLGKEMKLYF AQTLHIGSSH LAPPNPDKSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]		0.8 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WEY, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of RRM domain in calcipressin 1
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Related entities 1. calcipressin 1, : 1 : 37 entities Detail

Experiments performed 2 experiments Detail