BMRB: 10083

PDZ domain of human RIM2B

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Regulating synaptic membrane exocytosis protein 2

Entity

1. Regulating synaptic membrane exocytosis protein 2 (polymer, Thiol state: all free), 131 monomers, 13988.71 Da Detail

GSSGSSGHSH SDKHPVTWQP SKDGDRLIGR ILLNKRLKDG SVPRDSGAML GLKVVGGKMT ESGRLCAFIT KVKKGSLADT VGHLRPGDEV LEWNGRLLQG ATFEEVYNII LESKPEPQVE LVVSRSGPSS G

Formula weight

13988.71 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 99.0 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.0 % (1449 of 1463)	99.1 % (758 of 765)	99.1 % (561 of 566)	98.5 % (130 of 132)
Backbone	99.0 % (764 of 772)	98.5 % (269 of 273)	99.5 % (373 of 375)	98.4 % (122 of 124)
Sidechain	99.3 % (798 of 804)	99.4 % (489 of 492)	99.0 % (301 of 304)	100.0 % (8 of 8)
Aromatic	91.2 % (62 of 68)	91.2 % (31 of 34)	90.6 % (29 of 32)	100.0 % (2 of 2)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. PDZ domain

GSSGSSGHSH SDKHPVTWQP SKDGDRLIGR ILLNKRLKDG SVPRDSGAML GLKVVGGKMT ESGRLCAFIT KVKKGSLADT VGHLRPGDEV LEWNGRLLQG ATFEEVYNII LESKPEPQVE LVVSRSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.17 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFG, Strand ID: A Detail

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Release date

2008-08-13

Citation

PDZ domain of human RIM2B
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Regulating synaptic membrane exocytosis protein 2, : 1 : 6 : 37 entities Detail

Interaction partners 1. Regulating synaptic membrane exocytosis protein 2, : 1 interactors Detail

Experiments performed 2 experiments Detail